| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | HOWELL, Jayli | FR | Helias Catholic High School | NT |
| 3 | DELK, Lily | SR | Helias Catholic High School | NT |
| 4 | RIGGS, Elizabeth | JR | Wright City High School | 47.27 |
| 5 | SCHATZLER, Cylee | SO | St. Clair High School | 51.03 |
| 6 | AVILA, Jocelyn | FR | St. Clair High School | NT |
| 7 | VERRETT, Lilly | FR | St. Clair High School | NT |