| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | ROMO-VAZQUEZ, Jose | JR | New Haven High School | NT |
| 3 | STUDDARD, Nolan | FR | Sullivan High School | NT |
| 4 | CHAPMAN, John | SO | St. Clair High School | NT |
| 5 | HOOD, Ethan | FR | Helias Catholic High School | NT |
| 6 | COREY, Jeremy | SO | Wright City High School | NT |
| 7 | PRITCHETT, Elijah | FR | Sullivan High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | LEBISH, Gavin | SR | St. Francis Borgia High School | NT |
| 2 | POLITTE, Russell | SO | Sullivan High School | NT |
| 3 | FURDEK, Tyler | SO | St. Clair High School | NT |
| 4 | HUNN, Andrew | SO | Wright City High School | 1:05.79 |
| 5 | RIO, Gabriel | SR | St. Francis Borgia High School | 1:05.85 |
| 6 | BLAKE, Casen | FR | St. Clair High School | NT |
| 7 | ADOLPHSON, Joseph | SR | St. Francis Borgia High School | NT |
| 8 | BICKMEYER, Ethan | JR | St. Francis Borgia High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | RAY, Austin | FR | Sullivan High School | 1:00.36 |
| 2 | ARNDT, Noah | SO | St. Clair High School | 58.02 |
| 3 | BEAGLE, Zechariah | SO | Sullivan High School | 54.16 |
| 4 | KNERNSCHIELD, Harrison | JR | Helias Catholic High School | 51.92 |
| 5 | FISHER, Korbin | SR | Helias Catholic High School | 51.93 |
| 6 | CRISOSTOMO, Deven | JR | Helias Catholic High School | 55.22 |
| 7 | HAWKINS, Josh | SO | St. Clair High School | 1:00.09 |
| 8 | HAMILTON, Jack | SO | Helias Catholic High School | 1:03.58 |