| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | St. Francis Borgia High School | A | Dowil | Rondot | Jacobsen | Smith | NT |
| 3 | Wright City High School | A | NT | ||||
| 4 | Helias Catholic High School | B | Vallon | Mason | Jahnsen | Lock | 52.64 |
| 5 | Helias Catholic High School | A | Struemph | Dollar | French | Howell | 52.64 |
| 6 | Sullivan High School | A | Carpenter | Dace | Garner | Peterson | NT |
| 7 | Wright City High School | B | NT |