| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Wright City MS | A | Reimers | Schanuel | Thompson | Thompson | NT | Unverified: |
| 4 | Silex Middle School | A | Demitroff | Kuntz | Van Horn | Grateke | 2:13.53 | Verified |
| 5 | Winfield MS | A | Davidson | Holder | Malloy | Pund | 2:14.04 | Verified |
