| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Montgomery County MS | A | NT | Unverified: | ||||
| 3 | Elsberry Middle School | A | Hunter | Taylor | Smith | Hardwick | NT | Unverified: |
| 4 | Winfield MS | A | Holder | Kirkemeyer | Liberson | Pund | 1:00.45 | Verified |
| 5 | Silex Middle School | A | Demitroff | Kuntz | Van Horn | Harris | 1:02.78 | Verified |
| 6 | Wright City MS | A | Wilson | SIms | Meier | Ferrell | NT | Unverified: |
| 7 | Wright City MS | B | Reimers | Schanuel | Thompson | Thompson | NT | Unverified: |
