| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton Middle School | A | Waller | Hall | Wilson | Salmons | 1:00.13 | Verified |
| 3 | Winfield MS | A | Beck | Johnson | Marsten | Phelps | 52.69 | Verified |
| 4 | Black Hawk Middle School | A | Peth | Forrest | Jackson | McPike | 50.94 | Verified |
| 5 | Wright City MS | A | Baugher | Cross | Schnarre | Love | 51.36 | Verified |
| 6 | Winfield MS | B | Ash | Meyer | Rowland | Ruckel | 52.69 | Verified |
| 7 | St. Louis Blue Knights Middle Sc | A | Ward | Kusmanoff | Clipper | Gall | 1:10.78 | Verified |
