| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clopton Middle School | A | Adam | Enloe | Talbert | Caplin | 1:05.89 | Verified |
| 4 | St. Louis Blue Knights Middle Sc | A | Plymale | Funk | Stiles | Higgins | 1:03.10 | Verified |
| 5 | Clopton Middle School | B | Kaimannn | Craig | Heuer | Bankhead | 1:05.89 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Elsberry Middle School | A | Brinkman | Howard | Sifuentes | Taylor | 1:03.02 | Verified |
| 2 | Winfield MS | B | Hillen | Ohlms | Munie | Walcott | 58.00 | Verified |
| 3 | Wright City MS | A | Ferrell | Meier | SIms | Wilson | 57.03 | Verified |
| 4 | Silex Middle School | A | Harris | Mehler | Benton | Van Horn | 55.28 | Verified |
| 5 | Wright City MS | B | Reimers | Thompson | Thompson | Schanuel | 57.03 | Verified |
| 6 | Winfield MS | A | Holder | Liberson | Pund | VanCleve | 58.00 | Verified |
| 7 | Black Hawk Middle School | A | Vacek | Ruff | Allen | Sebastian | 1:00.48 | Verified |
