| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERRY, Kade | JR | Winfield High School | 5:37.37 | Verified |
| 2 | MCMILLEN, Megan | SR | Bowling Green High School | 5:55.00 | Verified |
| 3 | OBRIEN, Jerorgia | JR | Palmyra High School | 6:05.30 | Verified |
| 4 | GILKISON, Emma | JR | St. Charles High School | 6:19.78 | Verified |
| 5 | BOSCHERT, Lily | JR | Winfield High School | 6:39.84 | Verified |
| 6 | LAUER, Keeley | SO | St. Charles High School | 7:12.13 | Verified |
| 7 | LINDSAY, Carly | SO | Silex High School | 7:29.56 | Verified |
| 8 | WEITKAMP, Macy | SR | Clopton High School | 7:32.09 | Verified |
| 9 | CLABORN, MCKENNA | 9 | Silex High School | NT | Unverified: |
| 10 | NOTTMEYER, Ella | 9 | St. Charles High School | NT | Unverified: |
