| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | NELKE, Jersy | 9 | Winfield High School | NT | Unverified: |
| 4 | WELLS, Natalee | SO | Bowling Green High School | NT | Unverified: |
| 5 | KINION, CLAIRE | 9 | Silex High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | NASH, Taylor | SO | St. Charles High School | 1:01.90 | Verified |
| 3 | GILLESPIE, Kailey | JR | Winfield High School | 54.30 | Verified |
| 4 | OELLERMANN, Mia | SR | St. Charles High School | 47.69 | Verified |
| 5 | KRIGBAUM, Emma | JR | Palmyra High School | 52.92 | Verified |
| 6 | ZELLMER, Julia | SO | Winfield High School | 58.64 | Verified |
| 7 | JAMES, Ashley | 9 | Silex High School | NT | Unverified: |
