| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHANCE, Ben | SO | Bowling Green High School | 4:49.60 | Verified |
| 2 | MAPLES, Nate | SO | St. Charles High School | 4:50.30 | Verified |
| 3 | DODSON, Dashaun | JR | Elsberry High School | 4:52.30 | Verified |
| 4 | NOTTMEYER, Luke | SO | St. Charles High School | 4:55.00 | Unverified: |
| 5 | WARNER, James | SO | St. Charles High School | 4:56.20 | Unverified: |
| 6 | JUETTE, Caleb | SR | Palmyra High School | 5:14.23 | Verified |
| 7 | BROSS, Connor | JR | Palmyra High School | 5:25.49 | Verified |
| 8 | BURK, Micah | SO | Winfield High School | 6:03.90 | Verified |
| 9 | WRIGHT, Levi | SO | Winfield High School | 6:11.76 | Verified |
| 10 | GRADDY, Hunter | 9 | Winfield High School | NT | Unverified: |
| 11 | REID, Charles | 9 | St. Charles High School | NT | Unverified: |
| 12 | LEON, Roberto | 9 | Bowling Green High School | NT | Unverified: |
