| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BANKS, Tristen | SO | Parkway West High School | NT | Unverified: |
| 2 | SHANK, Jojo | SO | Parkway West High School | 23.40 | Unverified: |
| 3 | MARTZ, Kyrstiana | 9 | Kirkwood High School | 21.54 | Verified |
| 4 | ELGIN, Taylor | 9 | Nerinx Hall High School | 22.00 | Unverified: time trial |
| 5 | MACKINSON, Eleanor | JR | Lindbergh High School | 24.61 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARIC, Emma | 9 | Kirkwood High School | 20.88 | Verified |
| 2 | BURCH, Katelyn | JR | Mehlville High School | 19.57 | Verified |
| 3 | MURDOCK, Samantha | SO | Webster Groves High School | 18.07 | Verified |
| 4 | ROBERTS, Olivia | JR | Mehlville High School | 19.12 | Verified |
| 5 | GAINER, Micaiah | SR | Lindbergh High School | 20.17 | Verified |
| 6 | O'GORMAN, Lucy | SO | Nerinx Hall High School | 21.47 | Verified |
