| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Gateway Science Charter | A | 4:12.46 | Verified | ||||
| 3 | Mehlville High School | A | Utsey | Nasufovic | Ndiaye | Kaletka | DNS | Verified |
| 4 | Parkway West High School | A | Martin | Taylor | Li | Athman | 4:10.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Peel | Miller | Salmon | Weir | 3:55.73 | Unverified: |
| 2 | Kirkwood High School | A | Reynolds | Birmingham | Wallace | Robinson | NT | Unverified: |
| 3 | Ladue Horton Watkins High School | A | 3:41.61 | Unverified: | ||||
| 4 | Ritenour High School | A | Johnson | Whitehorn | Strickland | Kadima | 3:45.00 | Unverified: |
| 5 | Lindbergh High School | A | 3:54.17 | Unverified: | ||||
| 6 | St. Louis University High School | A | 4:00.60 | Unverified: |
