| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | EDWARDS, Amirah | SO | Incarnate Word Academy | NT | Unverified: |
| 3 | TREVISANO, TAYLOR | JR | Mehlville High School | 33.15 | Verified |
| 4 | SCHELLER, Thaxton | 9 | Nerinx Hall High School | 33.21 | Verified |
| 5 | BANNING, Ava | 9 | Lindbergh High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KRUEGER, Lily | SR | Lindbergh High School | 31.73 | Verified |
| 2 | KNAPP, Lauren | SO | Webster Groves High School | 31.08 | Verified |
| 3 | MAYS, Aryah | JR | Incarnate Word Academy | 30.60 | Verified |
| 4 | TROSKY, Allison | SR | Mehlville High School | 30.98 | Verified |
| 5 | PIERCE, Anna | 9 | Webster Groves High School | 31.65 | Unverified: |
| 6 | WALSH, Avery | SO | Nerinx Hall High School | 31.83 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BURGETT, Karla | SO | Kirkwood High School | 29.89 | Verified |
| 2 | ROGERS, Madison | SO | Ladue Horton Watkins High School | 29.50 | Verified |
| 3 | BANKS, Tristen | SO | Parkway West High School | 28.50 | Unverified: |
| 4 | ASHOK, Riya | SO | Parkway West High School | 29.50 | Unverified: |
| 5 | RAY, Kayla | 9 | Ladue Horton Watkins High School | 29.62 | Verified |
| 6 | PERKINS, Olivia | JR | Kirkwood High School | 29.95 | Verified |
