| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SCHELINSKI, Lilly | JR | Notre Dame (St. Louis) High Scho | NT | Unverified: |
| 3 | ELSTON, Brooke | JR | Rosati-Kain High School | 1:01.28 | Verified |
| 4 | SINGER, Rachel | JR | Villa Duchesne High School | NT | Unverified: |
| 5 | BUSSEN, Beatrice | SO | Villa Duchesne High School | NT | Unverified: |
