| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ultimate Speed Academy | B | Holmes | Robinson | Graham | Burns | NT | |
| 2 | Ultimate Speed Academy | A | Wilson | Freeman | Saldivar | Martin | NT | |
| 3 | St. Louis Saints | A | Durant | Durant | Thornton III | Turner | NT | |
| 6 | ST Louis Scorpions Track Club | A | Phelps | Harris | Moore | Evans | NT | |
| 7 | Blues Track Club of Greater St | A | Clayborn | Pruitt Jr | Williams Iii | Allen | NT |
