| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | ST Louis Scorpions Track Club | A | King | Hammond | Kerr | Whitaker | NT | |
| 2 | Ultimate Speed Academy | D | Rogers | Fleming | Maroney | Trevino | NT | |
| 3 | Blues Track Club of Greater St | B | Solomon | Graham | Johnson | White | NT | |
| 4 | Ultimate Speed Academy | B | Chunn | Higgins | Shim | Langford | NT | |
| 5 | Ultimate Speed Academy | C | Turner | Shim | Jennings | Moore | NT | |
| 6 | Ultimate Speed Academy | A | Jennings | Jones | Norwood | Sanders | NT | |
| 7 | Blues Track Club of Greater St | A | Hensgens | Madison | Dickerson | Lewis | NT |
