| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | ST Louis Scorpions Track Club | A | Williams | Fox | Word | Fox | NT | |
| 3 | St. Louis Saints | A | Matthews | Magee | Jones | Cooper | NT | |
| 4 | Ultimate Speed Academy | B | NT | |||||
| 5 | Ultimate Speed Academy | A | Collins | Graham | Hudson | Lewis Jr | NT | |
| 6 | Blues Track Club of Greater St | A | Hubbard | Salter | Robinson | James Jr | NT |
