| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Thunder Speed Track Club | A | Bester | Tipler | Zilliner | NT | ||
| 4 | Ultimate Speed Academy | B | Moore | Dye | Hodges | Williams Iii | NT | |
| 5 | ST Louis Scorpions Track Club | B | Donaldson | Smith | Potts | Fenton III | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | ST Louis Scorpions Track Club | A | Johnson | Courtney | Hayes | Settles | NT | |
| 3 | ST Louis Scorpions Track Club | C | Clay | Conway | Davis | Curtis | NT | |
| 4 | Ultimate Speed Academy | A | Ming | Riggins | Woods | Vincent Jr | 2:10.00 | |
| 5 | Ultimate Speed Academy | D | Lowe | Edwards | Thompson Jr | Gasama | NT | |
| 6 | Ultimate Speed Academy | C | Jackson | Thompson | Jenkins | Jackson | NT | |
| 7 | Blues Track Club of Greater St | A | Bealer | Clark | Lane | Houston | NT |
