| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Blues Track Club of Greater St | B | Arms | Murkey | Martin | Blockton | NT | |
| 2 | Blues Track Club of Greater St | A | Bryant | Kinsch-Mcfield | Jones | Billingsley | NT | |
| 3 | Ultimate Speed Academy | A | NT | |||||
| 6 | ST Louis Scorpions Track Club | A | Union | Tucker | Harkless | Hill | NT | |
| 7 | Thunder Speed Track Club | A | NT | |||||
| 8 | ST Louis Scorpions Track Club | B | Nunn | Sims | Young | Jefferson | NT |
