| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | ST Louis Scorpions Track Club | A | Echols | Settles III | Young | Harkless Jr | NT | |
| 2 | Top Speed Track Club STL | A | Reed Jr | Haywood | Long Jr | Johnson | NT | |
| 3 | Ultimate Speed Academy | A | Malcolm | Shim | Reeves | Betts | NT | |
| 4 | Blues Track Club of Greater St | A | Reed | Harrington | Brown | Patterson | NT | |
| 5 | Blues Track Club of Greater St | B | Johnson | Dukes | Young | Love | NT | |
| 6 | St. Louis Saints | A | Thornton | Reedus | Williams-Webb | Warren | NT | |
| 7 | Ultimate Speed Academy | B | NT |
