| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Blues Track Club of Greater St | A | Spencer Roberts | Clark | White | Carter | NT | |
| 3 | Ultimate Speed Academy | A | Ivy | Daniels | Williams | Murphy | NT | |
| 4 | ST Louis Scorpions Track Club | A | Thomas | Archie | Hammond | Taylor | NT | |
| 5 | Blues Track Club of Greater St | B | Fisher | Bass | Worshim-Mahone | Fisher | NT | |
| 6 | ST Louis Scorpions Track Club | B | Randle | Johnson | Curtis | Hammond | NT |
