| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 7 | JONES, Brooke | Ultimate Speed Academy | 2:29.22 | ||
| 8 | EATHERTON, Addison | Blue Thunder Track Club | 2:35.38 | ||
| 9 | JENKINS, Morgan | Capital Area Track 2 | 2:38.00 | ||
| 10 | ROGERS, Aaliyah | Ultimate Speed Academy | 2:38.59 | ||
| 11 | WHITE, Damia | Herbert Hoover | 2:49.61 | ||
| 12 | DAWSON, Mckenzie | Herbert Hoover | 2:57.61 | ||
| 13 | MAIN, Abigail | Ultimate Speed Academy | 3:01.76 | ||
| 14 | GROENEWALD, Marli | St. Louis Blazers Track Club | NT | ||
| 15 | GALLOWAY, Samaria | R.C Striders | NT | ||
| 16 | HIGGINS, Demetria | Ultimate Speed Academy | NT | ||
| 17 | JONES, Sydnee | St. Louis PAL | NT | ||
| 18 | HUBBARD, Joi | St. Louis PAL | NT | ||
| 19 | CANDE, Catherine | St. Louis Blazers Track Club | NT | ||
| 20 | MEEKS, Derriyah | Dashers Track and Sports | NT |
