| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAMPBELL, Gabrielle | Ultimate Speed Academy | 2:39.41 | ||
| 2 | CAMPBELL, Melanie | Ultimate Speed Academy | 2:52.44 | ||
| 3 | LONG, Bella | Blue Thunder Track Club | 3:08.15 | ||
| 4 | HOOVER, Maya | Blue Thunder Track Club | 3:10.74 | ||
| 5 | LEMEN, Eleanor | Blues Track Club of Greater St | NT | ||
| 6 | FERRONE, Emma | St. Louis Blazers Track Club | NT | ||
| 7 | WILLIAMS, Ha'Layna | St. Louis Saints | NT | ||
| 8 | MUELLER, Jordan | Ultimate Speed Academy | NT | ||
| 9 | JOHNSON, Aaliyah | Ultimate Speed Academy | NT | ||
| 10 | HOFFSUEMMER, Charlotte | St. Louis Blazers Track Club | NT | ||
| 11 | NOLAN, Norah | St. Louis Blazers Track Club | NT | ||
| 12 | TRAUGHBER, Hannah | St. Louis Blazers Track Club | NT |
