| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 13 | BEATTY, Aaliyah | Ultimate Speed Academy | 3:02.70 | ||
| 14 | MCLAURIN, Jordynn | Herbert Hoover | 3:25.76 | ||
| 15 | GRILLIOT, Lana | St. Louis Blazers Track Club | NT | ||
| 16 | BLOCKTON, Myla | Blues Track Club of Greater St | NT | ||
| 17 | RANDALL, Fallon | Ultimate Speed Academy | NT | ||
| 18 | HOFFMAN, Destani | Road Runners Express | NT | ||
| 19 | HOFFSUEMMER, Eleanor | St. Louis Blazers Track Club | NT | ||
| 20 | MURKEY, Cassidy | Blues Track Club of Greater St | NT | ||
| 21 | RONNIE, Amelie | St. Louis Blazers Track Club | NT | ||
| 22 | MASSEY, Jakyra | St. Louis Saints | NT | ||
| 23 | JEFFERSON, Kamryn | ST Louis Scorpions Track Club | NT | ||
| 24 | NOUGUES, Keira | Capital Area Track 2 | NT |
