| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GRIEVE, Kai | Capital Area Track 2 | 3:30.00 | ||
| 2 | THORNTON, Adonis | St. Louis Saints | NT | ||
| 3 | ECHOLS, Chase | ST Louis Scorpions Track Club | NT | ||
| 4 | NEELY, Alexander | Dashers Track and Sports | NT | ||
| 5 | SETTLES III, Geno | ST Louis Scorpions Track Club | NT | ||
| 6 | MOORE, Michael | St.Charles Heat | NT | ||
| 7 | WARREN, Makhi | St. Louis Saints | NT | ||
| 8 | STEVENSON, King | Herbert Hoover | NT | ||
| 9 | AARON, Titan | Ultimate Speed Academy | NT | ||
| 10 | WESLEY, Laden | Herbert Hoover | NT | ||
| 11 | WILSON, David | Ultimate Speed Academy | NT | ||
| 12 | LITTLEJOHN, Michael | Herbert Hoover | NT | ||
| 13 | DORSEY III, Marcus | St. Louis PAL | NT | ||
| 14 | FISCHER, Daniel | Missouri Elite Sports Club | NT | ||
| 15 | BETTS, Jaydan | Ultimate Speed Academy | NT |
