| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHIM, Demiah | Ultimate Speed Academy | NM | ||
| 2 | LANGE, Jordan | Jets Track Club | NM | ||
| 3 | SHIM, Demi | Ultimate Speed Academy | NM | ||
| 4 | GALLOWAY, Samaria | R.C Striders | NM | ||
| 5 | MADISON, Bryaira | Blues Track Club of Greater St | NM | ||
| 6 | MOPKINS, Armayaih | Herbert Hoover | NM | ||
| 7 | JENNINGS, London | Ultimate Speed Academy | NM | ||
| 8 | GRAY, Ariah | Top Speed Track Club STL | NM | ||
| 9 | BENNETT, Akili | Tallawah Track and Field | NM | ||
| 10 | BOHANNON, Tamara | Missouri Elite Sports Club | NM | ||
| 11 | TOLAR, Samaria | Dashers Track and Sports | NM | ||
| 12 | WORTHY, Makena | St. Louis Saints | NM | ||
| 13 | LANGFORD, Kamryn | Ultimate Speed Academy | NM | ||
| 14 | DAWSON, Mckenzie | Herbert Hoover | NM | ||
| 15 | HENSGENS, Bailey | Blues Track Club of Greater St | NM | ||
| 16 | CHUNN, Calise | Ultimate Speed Academy | NM | ||
| 17 | BUCHANAN, I'Ahnna | Missouri Elite Sports Club | NM | ||
| 18 | TURNER, Ameria | Ultimate Speed Academy | NM | ||
| 19 | KERR, Na'ila | ST Louis Scorpions Track Club | NM | ||
| 20 | TURNTINE, Christin | Tallawah Track and Field | NM | ||
| 21 | JENNINGS, Kendra | Thunder Speed Track Club | NM | ||
| 22 | JENNINGS, Aniyah | Ultimate Speed Academy | NM | ||
| 23 | ALLEN, Lauren | ST Louis Scorpions Track Club | NM | ||
| 24 | REDDING, Sasha | St. Louis Saints | 4.10m | ||
| 25 | EATHERTON, Addison | Blue Thunder Track Club | 4.16m | ||
| 26 | MALAND, Claire | Blue Thunder Track Club | 5.03m |
