| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOODHOUSE, Jewel | Road Runners Express | NM | ||
| 2 | SESSION, Frankii | Missouri Elite Sports Club | NM | ||
| 3 | LEWIS, Marley | Blues Track Club of Greater St | NM | ||
| 4 | DOBBINS, Destinee | Dashers Track and Sports | NM | ||
| 5 | GATES, Kyleigh | Dashers Track and Sports | NM | ||
| 6 | BELL, Allana | Unattached | NM | ||
| 7 | LANGFORD, Kyndall | Ultimate Speed Academy | NM | ||
| 8 | COLEMAN, Zoe | St. Louis PAL | NM | ||
| 9 | SCOTT, Jordynn | Ultimate Speed Academy | NM | ||
| 10 | MARTIN, Zaryiah | ST Louis Scorpions Track Club | NM | ||
| 11 | WILLIAMS, Syriah | Dashers Track and Sports | NM | ||
| 12 | WILLIAMS, Jazelle | Road Runners Express | NM | ||
| 13 | WILSON, Olivia | St.Charles Heat | NM | ||
| 14 | MERRIWEATHER, Dallas | U. City Xplosion Track & Field | NM | ||
| 15 | WILLIAMS, Ha'Layna | St. Louis Saints | NM | ||
| 16 | RAY, Janiya | St. Louis Saints | NM |
