| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TOWNS, K'Myla | Thunder Speed Track Club | NM | ||
| 2 | YOUNG, Ka'Lyce | ST Louis Scorpions Track Club | NM | ||
| 3 | HOFFMAN, Destani | Road Runners Express | NM | ||
| 4 | MEADOWS, Olivia | Missouri Elite Sports Club | NM | ||
| 5 | ROBINSON, Qenai | Blue Thunder Track Club | NM | ||
| 6 | WILLIAMS, Trinity | Dashers Track and Sports | NM | ||
| 7 | ASH, Aubree | Road Runners Express | NM | ||
| 8 | RANDALL, Fallon | Ultimate Speed Academy | NM | ||
| 9 | COLEMAN, Hailey | St. Louis PAL | NM | ||
| 10 | MCLAURIN, Jordynn | Herbert Hoover | NM | ||
| 11 | MARTIN, Cheyenne | Blues Track Club of Greater St | NM | ||
| 12 | WILLIAMS, Jocelyn | Road Runners Express | NM | ||
| 13 | STANCIEL, Bella-Chanel | Ultimate Speed Academy | NM | ||
| 14 | UNION, Casey | ST Louis Scorpions Track Club | NM | ||
| 15 | FINLEY, Kaylee | ST Louis Scorpions Track Club | NM | ||
| 16 | KINSCH-MCFIELD, Kassidi | Blues Track Club of Greater St | 3.30m |
