| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUDSON, Jordynn | St. Louis Saints | NM | ||
| 2 | LIDDELL, Tamiya | Road Runners Express | NM | ||
| 3 | MALCOLM, Aria | Ultimate Speed Academy | NM | ||
| 4 | WARREN, Mckinley | St. Louis Saints | NM | ||
| 5 | DUNN, Celeste | Dashers Track and Sports | NM | ||
| 6 | CROSBY, Keisera | Blues Track Club of Greater St | NM | ||
| 7 | CRONAN, Caroline | Tallawah Track and Field | NM | ||
| 8 | BRASELMAN, Caylee | St. Louis Saints | NM | ||
| 9 | GALLO, Ellie | Tallawah Track and Field | NM | ||
| 10 | FREEMAN, Zoey | Ultimate Speed Academy | NM | ||
| 11 | BROWN, Jailah | Road Runners Express | NM | ||
| 12 | CLARK, Azraa | Blue Thunder Track Club | NM | ||
| 13 | LEWIS, Sherrell | Blue Thunder Track Club | NM | ||
| 14 | SCOTT, Selena | Tallawah Track and Field | NM | ||
| 15 | HAAR, Autumn | Tallawah Track and Field | NM | ||
| 16 | FIRST, Marley | Herbert Hoover | NM | ||
| 17 | IVY, Kaionni | Ultimate Speed Academy | NM | ||
| 18 | JONES, Talia | Missouri Elite Sports Club | NM | ||
| 19 | SHERMAN, Calise | Top Speed Track Club STL | NM | ||
| 20 | BUCHANAN, Koi | R.C Striders | NM | ||
| 21 | COMBS, Dream | Road Runners Express | NM | ||
| 22 | DONALD, Da'Layna | Thunder Speed Track Club | NM | ||
| 23 | BRASELMAN, Chlo | St. Louis Saints | NM | ||
| 24 | DAVIS, Lylah | Road Runners Express | NM |
