| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt East High School | A | Williams | Franklin | Rudd | James | 1:42.72 | Verified |
| 4 | Winfield High School | A | Hyde | Riley | Trempler | Baldwin | 1:36.93 | Verified |
| 5 | St. Charles High School | A | Mbroh | Chang | Murphy | Calmese | 1:37.99 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell High School | A | Davis | Wilmes | Mais | Deal | 1:35.00 | Unverified: Todd Bizzell Memorial "Spartan R |
| 3 | Troy Buchanan High School | A | Giacopelli | Ryan | Hall | Benhardt | 1:33.65 | Verified |
| 4 | Hannibal High School | A | Porter | Robertson | Bergheger | Williams | 1:32.07 | Verified |
| 5 | Orchard Farm High School | A | Lewis | Williams | Jewell | Moore | NT | Unverified: Orchard Farm Eagle Invite |
| 6 | Warrenton High School | A | Meine | Bristol | Reel | Evans | 1:34.57 | Verified |
| 7 | Ft. Zumwalt South High School | A | Raife | Bates | Dewitt | Pilger | DQ | Verified |
