| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Orchard Farm High School | A | Ditmeyer | Shields | Jones | Jewell | NT | Verified |
| 2 | St. Charles High School | A | Walker | Hollrah | O'Keeffe | Long | 1:56.60 | Unverified: |
| 3 | Hannibal High School | A | Williams | Dean | Thompson | Scott | 1:52.72 | Verified |
| 4 | Troy Buchanan High School | A | Crouch | Hunolt | Starkey | Robinson | 1:50.78 | Verified |
| 5 | Ft. Zumwalt South High School | A | Moore | Clark | Douglas | Burnett | NT | Verified |
| 6 | Winfield High School | A | Turnbull | Stark | Reinebach | Reinebach | 1:56.03 | Verified |
| 7 | Warrenton High School | A | Overkamp | Marschel | Love | Rhama | 1:57.10 | Verified |
