| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles High School | A | Oellermann | Long | Gilkison | O'Keeffe | 4:34.13 | Verified |
| 3 | Ft. Zumwalt South High School | A | Clark | Voss | Brauch | Whitten | 4:32.68 | Verified |
| 4 | St. Charles West High School | A | Nichols | Zeier | Myers | Jackson | 4:20.62 | Verified |
| 5 | Hannibal High School | A | Dorsey | Groth | Williams | Scott | 4:32.63 | Verified |
| 6 | Troy Buchanan High School | A | Brown | Ellison | Cook | Getz | 4:33.95 | Verified |
| 7 | Winfield High School | A | Reinebach | Boschert | Ford | Berry | 4:38.15 | Verified |
