| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WEAVER, Elinor | SO | Troy Buchanan High School | NT | |
| 3 | KINNER, Taylor | SR | St. Charles High School | 32.32 | Verified |
| 4 | SPIVEY, Nylah | JR | Ft. Zumwalt East High School | 30.93 | Verified |
| 5 | BRAND, Ava | JR | St. Clair High School | 30.94 | Verified |
| 6 | WALTON, Alexis | SO | Francis Howell High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HACKMANN, Abigail | JR | Warrenton High School | 30.49 | Verified |
| 2 | MOORE, Vada | JR | St. Clair High School | 29.82 | Verified |
| 3 | JONES, Bella | 9 | Orchard Farm High School | 29.45 | Verified |
| 4 | BOXDORFER, Aaliyah | JR | St. Charles West High School | 28.76 | Verified |
| 5 | WALKER, Nakiyah | SO | St. Charles High School | 28.96 | Verified |
| 6 | SHIVERS, Jillian | SR | St. Charles West High School | 29.50 | Verified |
| 7 | JEWELL, Dyamond | JR | Orchard Farm High School | 30.45 | Verified |
| 8 | SCHIERMEIER, Khyla | JR | Francis Howell High School | 30.81 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OVERKAMP, Kylie | SO | Warrenton High School | 28.42 | Verified |
| 2 | DEAN, Sameya | 9 | Hannibal High School | 28.18 | Verified |
| 3 | CLARK, Inaiah | SO | Ft. Zumwalt South High School | 28.10 | Verified |
| 4 | BOBEEN, Breana | SO | Troy Buchanan High School | 27.43 | Verified |
| 5 | BURNETT, Nia | SR | Ft. Zumwalt South High School | 27.80 | Verified |
| 6 | SCOTT, Avion | SR | Hannibal High School | 28.12 | Unverified: |
| 7 | REINEBACH, Grace | JR | Winfield High School | 28.29 | Verified |
| 8 | STARK, Evelyn | 9 | Winfield High School | 28.50 | Verified |
