| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | FISCHER, Nathan | JR | Orchard Farm High School | 47.47 | Verified |
| 3 | MILAM, Ethan | JR | Francis Howell High School | 45.73 | Verified |
| 4 | HYDE, Matthew | SO | Winfield High School | 45.07 | Verified |
| 5 | SPROUSE, Tanner | JR | Francis Howell High School | 45.64 | Verified |
| 6 | BOURGEADE, Luc | JR | St. Charles High School | 46.24 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUMPHREY, Markahl | JR | Hannibal High School | 44.50 | Verified |
| 2 | POWELL, Jacobbie | SR | Ft. Zumwalt South High School | 43.56 | Verified |
| 3 | LARUE, Lucas | SO | Troy Buchanan High School | 43.40 | Verified |
| 4 | NENNERT, Charles | SR | St. Charles High School | 42.39 | Verified |
| 5 | DEWITT, Xander | SO | Ft. Zumwalt South High School | 42.54 | Verified |
| 6 | EDWARDS, Harrison | JR | Troy Buchanan High School | 43.43 | Verified |
| 7 | BALDWIN, Xavier | SR | Winfield High School | 44.06 | Verified |
| 8 | JURADO, Joseph | SO | Hannibal High School | 44.93 | Verified |
