| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Zora | SR | Francis Howell High School | NT | Unverified: |
| 2 | NASH, Taylor | SO | St. Charles High School | 59.40 | Verified |
| 3 | DANIELS, Emelia | 9 | Troy Buchanan High School | 55.52 | Verified |
| 4 | THOMPSON, Jorie | SO | Hannibal High School | 54.09 | Verified |
| 5 | GILLESPIE, Kailey | JR | Winfield High School | 54.65 | Verified |
| 6 | SCHLEUTER, Kiran | SR | Warrenton High School | 57.95 | Unverified: |
| 7 | MOORE, Serena | JR | Ft. Zumwalt South High School | 1:08.81 | Verified |
| 8 | RASMUSSEN, Tina | JR | St. Clair High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARK, Nora | JR | Hannibal High School | 53.21 | Verified |
| 2 | SCHATZLER, Cylee | 9 | St. Clair High School | 52.20 | Verified |
| 3 | NELKE, Jersy | 9 | Winfield High School | 51.65 | Verified |
| 4 | DOUGLAS, Triniti | JR | Ft. Zumwalt South High School | 50.00 | Verified |
| 5 | OELLERMANN, Mia | SR | St. Charles High School | 51.31 | Verified |
| 6 | SPANN, Carlie | JR | Ft. Zumwalt East High School | 51.86 | Verified |
| 7 | PANUS, Paige | SR | Francis Howell High School | 52.37 | Verified |
| 8 | BROWN, Teryn | SO | Troy Buchanan High School | 53.73 | Verified |
