| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Francis Howell High School | A | Shinabery | Price | Stewart | Mattingly | 10:04.38 | Verified |
| 2 | St. Charles West High School | A | Jackson | Zeier | Jones | Jackson | NT | Verified |
| 3 | Ft. Zumwalt South High School | A | Voss | Morrison | Hart | Holman | 10:54.69 | Verified |
| 4 | Warrenton High School | A | Marschel | Morris | Howard | Marschel | 11:15.17 | Verified |
| 5 | St. Charles High School | A | Gilkison | Rayburn | Nottmeyer | O'Keeffe | 11:16.02 | Verified |
| 6 | Hannibal High School | A | Griffen | Waelder | Bates | Bonvillian | 11:43.00 | Verified |
