| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rockwood Summit High School | A | Pitts | Emmenegger | Wallace | Taylor | 1:34.53 | Verified |
| 3 | Ladue Horton Watkins High School | A | 1:33.58 | Verified | ||||
| 4 | Parkway West High School | A | 1:29.03 | Verified | ||||
| 5 | McCluer High School | A | Mosley | McCoy | Greenlee | Bills | DQ | Verified |
| 6 | Riverview Gardens High School | A | Abel | Carlock | Hodges | Thompson | 1:33.87 | Verified |
| 7 | Parkway Central High School | A | Ellis | Banks | Cook | Walker | 1:35.32 | Verified |
