| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ladue Horton Watkins High School | A | DQ | Unverified: | ||||
| 4 | McCluer High School | A | Johnson | Moore | Walker | Jones | 1:32.47 | Verified |
| 5 | Rockwood Summit High School | A | Stevens | Grimes | Zerr | RUGGERI | DQ | Verified |
| 6 | Parkway Central High School | A | Ruckstaetter | Stoll | Sharma | Brown | 1:43.40 | Unverified: |
