| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Parkway Central High School | A | Stoll | Brown | Mraz | Moresi | 3:53.60 | Unverified: |
| 3 | Ladue Horton Watkins High School | A | 3:43.92 | Unverified: | ||||
| 4 | Rockwood Summit High School | A | Zerr | Bordner | Grimes | Osterman | 3:38.02 | Verified |
| 5 | McCluer North High School | A | Thompson | Rimmer | Davis | Wilson | 3:40.73 | Verified |
| 6 | Parkway West High School | A | 3:45.00 | Unverified: | ||||
| 7 | McCluer High School | A | Howard | Williams | Cade | Middleton | NT | Unverified: |
