| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALLEN, Eric | JR | Parkway Central High School | 33.90 | Unverified: |
| 4 | BAYLESS, Chase | 9 | Riverview Gardens High School | NT | Unverified: |
| 5 | WALKER, Detroit | SO | McCluer High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HIPP, Max | SO | Parkway West High School | NT | Unverified: |
| 2 | MOORE, Lavon | SO | McCluer High School | 28.46 | Verified |
| 3 | ISERMANN, Cameron | 9 | Parkway Central High School | 27.80 | Unverified: |
| 4 | COLEMAN, Adam | SO | McCluer North High School | 26.53 | Verified |
| 5 | UELSMANN, Grant | SO | Rockwood Summit High School | 26.94 | Verified |
| 6 | JOHNSON, Nehemiah | 9 | McCluer High School | 28.41 | Verified |
| 7 | VERA, Esteban | SR | Parkway Central High School | 29.46 | Verified |
| 8 | BIBBS, Jacobie | SO | Riverview Gardens High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Jacob | 9 | McCluer North High School | 24.43 | Verified |
| 2 | STORMAN, Chase | 9 | Ladue Horton Watkins High School | 24.30 | Unverified: |
| 3 | STEVENS, Elijah | SO | Rockwood Summit High School | 23.84 | Verified |
| 4 | VU, Samuel | SO | Rockwood Summit High School | 23.61 | Verified |
| 5 | VIA, Gavin | SO | Ladue Horton Watkins High School | 23.77 | Verified |
| 6 | LEVINE, Owen | SO | Ladue Horton Watkins High School | 24.26 | Verified |
| 7 | STRAUSS, Jules | 9 | Parkway West High School | 24.36 | Verified |
| 8 | JONES, Santorus | SR | Riverview Gardens High School | 24.92 | Verified |
