| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HUGHES, MAKIYE | 9 | Rockwood Summit High School | 54.03 | Verified |
| 4 | MIDDLETON, David | SO | McCluer High School | 51.66 | Verified |
| 5 | FENG, Ryan | JR | Parkway West High School | 52.06 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PINEX, Jeremiah | 9 | Ladue Horton Watkins High School | 49.45 | Verified |
| 3 | FOWLER, KAMRON | 9 | Rockwood Summit High School | 48.24 | Verified |
| 4 | MRAZ, Jackson | SO | Parkway Central High School | 46.49 | Verified |
| 5 | STOLL, Aidan | JR | Parkway Central High School | 46.88 | Verified |
| 6 | HAWTHORNE, David | SO | Ladue Horton Watkins High School | 49.02 | Verified |
| 7 | RAVINDRANATH, Grisham | 9 | Ladue Horton Watkins High School | 49.93 | Verified |
