| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ladue Horton Watkins High School | A | 45.03 | Verified | ||||
| 3 | Rockwood Summit High School | A | Pitts | Emmenegger | Wallace | Taylor | 44.39 | Verified |
| 4 | Parkway West High School | A | 43.27 | Verified | ||||
| 5 | McCluer High School | A | Reynolds | McCoy | Gillespie | Bills | 44.01 | Verified |
| 6 | Parkway Central High School | A | Banks | Badhe | Krieger | Jackson | 44.87 | Verified |
| 7 | Riverview Gardens High School | A | Abel | Carlock | Hodges | Howard | NT | Verified |
