| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway Central High School | A | Ruckstaetter | Stoll | Sharma | Mraz | 47.20 | Unverified: |
| 4 | McCluer High School | A | Jones | McKinnie | Johnson | Walker | 44.01 | Verified |
| 5 | Rockwood Summit High School | A | Stevens | Grimes | Zerr | Vu | 44.39 | Verified |
| 6 | Ladue Horton Watkins High School | A | 48.03 | Unverified: |
