| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | DIALLO, Mawdo | 9 | John Burroughs High School | NT | Unverified: |
| 5 | STURMOSKI, Ethan | SR | Clayton High School | NT | Unverified: |
| 6 | GARCIA, Dylan | SR | Clayton High School | NT | Unverified: |
| 7 | CRAIG-MEYER, Dean | 9 | Lutheran South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PATEL, Shiv | 9 | Clayton High School | NT | Unverified: |
| 2 | HUDSON, Luke | SR | St. Louis Blue Knights | 1:02.67 | Verified |
| 3 | GATHUNGU, Jessie | JR | Principia High School | 54.87 | Verified |
| 4 | GREEN, Joseph | 9 | St. Ignatius College Prep High S | 52.31 | Verified |
| 5 | ZUKIC, Altan | JR | Fox High School | 53.36 | Verified |
| 6 | SAVOYE, Lucas | SR | Principia High School | 57.34 | Verified |
| 7 | GANESH, Vikram | JR | Principia High School | 1:03.72 | Verified |
| 8 | CRAIG-MEYER, Mitchell | JR | Lutheran South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAMPBELL, Deo | 9 | St. Ignatius College Prep High S | 51.68 | Verified |
| 2 | WARD, Justin | SR | Lutheran South High School | 49.76 | Verified |
| 3 | SCHRIMP, Keaton | 9 | Russellville High School | 47.41 | Verified |
| 4 | PISONI, Timothy | JR | Fox High School | 46.06 | Verified |
| 5 | CAPAZO, Anakin | SO | Russellville High School | 47.39 | Verified |
| 6 | PALUMBO, Nathan | SR | St. Louis Blue Knights | 47.73 | Verified |
| 7 | KIRKPATRICK, Kelan | 9 | Fox High School | 51.37 | Unverified: Devon Ford Relays |
| 8 | NOTZ, Jordan | SR | St. Louis Blue Knights | 52.16 | Verified |
