| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WAGSTAFF, Elise | 9 | Affton High School | NT | Unverified: |
| 4 | KARAHASANOVIC, Irma | 9 | Affton High School | NT | Unverified: |
| 5 | MCAULEY, Ella | SO | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WOLFE, Chloe | SO | Clayton High School | NT | Unverified: |
| 3 | NGUYEN, Sophia | 9 | St. Louis Blue Knights | 1:06.83 | Verified |
| 4 | ANDERSON, Devon | 9 | Lutheran South High School | 1:02.63 | Verified |
| 5 | MAJORS, Emily | 9 | St. Louis Blue Knights | 1:03.44 | Verified |
| 6 | SHAFFER, Michelle | 9 | Affton High School | 1:10.20 | Unverified: 10th Annual Bob G. Brunette Invi |
| 7 | WORNOR, Thelma | 9 | Lutheran South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WANG, Esther | JR | Clayton High School | 1:00.31 | Verified |
| 2 | MOORE, Madison | 9 | John Burroughs High School | 57.86 | Verified |
| 3 | COOMBS, Cali | JR | Russellville High School | 55.39 | Verified |
| 4 | GRIFFIN, Maeve | 9 | St. Ignatius College Prep High S | 55.31 | Verified |
| 5 | HEROD, Abby | SR | St. Louis Blue Knights | 55.34 | Verified |
| 6 | WATSON, Haily | JR | Lutheran South High School | 56.67 | Verified |
| 7 | BROWN, Sophia | SO | John Burroughs High School | 59.53 | Verified |
| 8 | MOORE, Gabrielle | 9 | John Burroughs High School | 1:01.37 | Verified |
