| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Whitfield High School | A | Wilkins | Al-sagr | Mckown | Asikainen | NT | Unverified: |
| 4 | Principia High School | A | Dow | Henderson | James | Jones | NT | Unverified: |
| 5 | St. Ignatius College Prep High S | A | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lutheran South High School | A | Labbee | Marsala | Rabe | Huebner | NT | Unverified: |
| 2 | Clayton High School | A | Ross | Baum | Chung | Barnils Prats | NT | Unverified: |
| 3 | Russellville High School | A | Brautigam | Stewart | Klatt | Potts | 5:01.23 | Unverified: |
| 4 | MICDS | A | Brothers | Zar | Gira | Coppel | 4:56.48 | Unverified: |
| 5 | St. Charles West High School | A | Shivers | Boxdorfer | Everley | Mccune | 5:00.34 | Verified |
| 6 | St. Louis Blue Knights | A | Stiles | Majors | Bryant | Bryant | NT | Unverified: |
| 7 | Fox High School | A | Schrempf | Rogers | Schepers | Alidani | NT | Unverified: |
