| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | EVERS, Benjamin | JR | Lindenwood | 1:00.06 | Dick Cochran Classic 2021 (4/23/2021) |
| 4 | SAMUELS, Rasheed | JR | Lindenwood | 56.85 | |
| 5 | SONNTAG, Tommy | JR | Lindenwood | 58.36 | Missouri S&T Miner Invitational (3/27/2021) |
| 6 | BAKER, James | SO | Saint Louis | NT |
