| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Saint Louis | B | Doncevic | Kieffer | Monson | Rust | 4:02.18 | Atlantic 10 Indoor Track & Field Championships (2/26/2022) |
| 2 | Saint Louis | A | Nichols | McDowell | Kieffer | Murray | 4:02.18 | Atlantic 10 Indoor Track & Field Championships (2/26/2022) |
| 3 | Maryville (Mo.) | A | Allen | Berberich | Courtois | Catic | 4:05.53 | GLVC Outdoor Championships (5/06/2021) |
| 4 | Bradley | A | Bryant | Koontz | Henderson | Dalton | 3:46.17 | Missouri Valley Championships (5/14/2021) |
| 5 | Missouri State | A | Ramos | Obazughanmwen | Henry | Ifah | 3:51.60 | Missouri Valley Championships (5/14/2021) |
