| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EASTHAM, Sophie | SO | Health Sciences and Pharmacy | 5:46.03 | AMC Conference Championships (5/07/2021) |
| 2 | SHERRILL, Mikayla | JR | Health Sciences and Pharmacy | 5:50.06 | Greenville University Select Meet (4/17/2021) |
| 3 | NIKLAUS, Ashleigh | SO | Saint Louis | NT | |
| 4 | GIGER, Taylor | FR | Missouri State | NT | |
| 5 | LOMBARDO, Anna | FR | Missouri State | NT | |
| 6 | GHORMLEY, Kylie | FR | Missouri State | NT | |
| 7 | QUICK, Taylor | FR | Saint Louis | NT | |
| 8 | O'BRIEN, Margaret | JR | Saint Louis | NT | |
| 9 | WOLF, Sara | FR | Saint Louis | NT | |
| 10 | LOURENS, Riley | SO | Missouri State | NT | |
| 11 | VOELKER, Kayla | FR | Missouri State | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SEAR, Carly | SO | Saint Louis | 4:58.00 | |
| 2 | HARRIS, Mary Margaret | FR | Missouri State | 5:00.00 | |
| 3 | WAKELAND, Allison | SR | Health Sciences and Pharmacy | 5:04.87 | AMC Conference Championships (5/07/2021) |
| 4 | CARR, Sarah | JR | Unattached | 5:05.00 | Billiken Invitational (3/25/2022) |
| 5 | DEVANTIER, Lea | SO | Saint Louis | 5:06.69 | Tom Botts Invitational (4/09/2021) |
| 6 | HAMRE, Alexandra | SO | Missouri State | 5:06.90 | 13th Annual David Suenram Gorilla Classic (4/10/2021) |
| 7 | HUFFMAN, Kirsten | SO | Lindenwood | 5:07.48 | Missouri S&T Miner Invitational (3/27/2021) |
| 8 | REID, Courtney | SO | Lindenwood | 5:07.99 | Missouri S&T Miner Invitational (3/27/2021) |
| 9 | LESIOTIS, Athena | SR | Quincy | 5:09.49 | SBU Invite (4/02/2021) |
| 10 | NEA, Margo | SO | Missouri State | 5:14.84 | 13th Annual David Suenram Gorilla Classic (4/10/2021) |
| 11 | CLAYTON, Natalie | SO | Missouri State | 5:35.94 | 13th Annual David Suenram Gorilla Classic (4/10/2021) |
| 12 | SHERWOOD, Laura | SR | Maryville (Mo.) | 5:40.70 | Dick Cochran Classic 2021 (4/23/2021) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Sierra | SO | Missouri State | 4:30.57 | Missouri Valley Championships (5/14/2021) |
| 2 | DAR, Edna | JR | Missouri State | 4:33.70 | Missouri Valley Championships (5/14/2021) |
| 3 | HARP, Olivia | SO | Bradley | 4:42.15 | Gibson Invitational sponsored by the Terre Haute CVB (4/16/2021) |
| 4 | GARRETT, Meagn | FR | Unattached | 4:44.00 | Billiken Invitational (3/25/2022) |
| 5 | ROLEWICZ, Ashley | JR | Missouri State | 4:44.95 | 2021 Joey Haines Invite (4/02/2021) |
| 6 | MCKOY, Claudrice | JR | Missouri State | 4:45.00 | |
| 7 | STRAUSS, Lily | SO | Bradley | 4:49.03 | Billiken Invitational (3/26/2021) |
| 8 | LESHER, Savannah | FR | Saint Louis | 4:50.00 | |
| 9 | MARCO, Nicole | SO | Saint Louis | 4:51.60 | Atlantic 10 Outdoor Track & Field Championships (5/01/2021) |
| 10 | MORIN, Sonja | FR | Saint Louis | 4:55.00 | |
| 11 | ROELOFSE, Lyndi | FR | Saint Louis | 4:55.00 | |
| 12 | PALMER, Shelby | JR | Missouri State | 4:56.93 | 13th Annual David Suenram Gorilla Classic (4/10/2021) |
