| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Missouri State | A | Rayford | Henry | Obazughanmwen | Ramos | 45.25 | Missouri Valley Championships (5/14/2021) |
| 5 | Saint Louis | A | Jones | Robinson | Andorf | Hasken | 48.06 | Atlantic 10 Outdoor Track & Field Championships (5/01/2021) |
